List of pUGW-based BiFC vectors

Vector name Gateway cassette Type Accession number
pnCGW nCFP-attR1-Cmr-ccdB-attR2 nCFP AB830538
pcCGGW cCFP(cGFP)-attR1-Cmr-ccdB-attR2 cCGFP AB830536
pGWnC attR1-Cmr-ccdB-attR2-nCFP nCFP AB830543
pGWcCG attR1-Cmr-ccdB-attR2-cCFP(cGFP) cCGFP AB830541
pnGGW nGFP-attR1-Cmr-ccdB-attR2 nGFP AB830539
pGWnG attR1-Cmr-ccdB-attR2-nGFP nGFP AB830544
pnYGW nYFP-attR1-Cmr-ccdB-attR2 nYFP AB626694
pcYGW cYFP-attR1-Cmr-ccdB-attR2 cYFP AB626696
pGWnY attR1-Cmr-ccdB-attR2-nYFP nYFP AB626693
pGWcY attR1-Cmr-ccdB-attR2-cYFP cYFP AB626695
pnRGW nRFP-attR1-Cmr-ccdB-attR2 nRFP AB830540
pcRGW cRFP-attR1-Cmr-ccdB-attR2 cRFP AB830537
pGWnR attR1-Cmr-ccdB-attR2-nRFP nRFP AB830545
pGWcR attR1-Cmr-ccdB-attR2-cRFP cRFP AB830542
The backborn of these bectors is derived from pUC119. Therefore, ampicillin can be used for selection of transformatns after LR recombiantion reaction. Since the nucleotide sequences of C-terminal CFP and C-terminal GFP are identical, the fragment is designated as cCG.


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